Our contributions in last years

Journal publications

2021

  1. Machine Learning of Analytical Electron Density in Large Molecules Through Message-Passing. Cuevas-Zuviría, B.; Pacios, L. F. Journal of Chemical Information and Modeling 2021, acs.jcim.1c00227. doi: 10.1021/acs.jcim.1c00227.
  2. New Insights into the Sensitization to Nonspecific Lipid Transfer Proteins from Pollen and Food: New Role of Allergen Ole e 7. Oeo‐Santos, C.; Navas, A.; Benedé, S.; Ruíz‐León, B.; Díaz‐Perales, A.; Vogel, L.; Moreno‐Aguilar, C.; Jurado, A.; Villalba, M.; Barderas, R. Allergy 2020, 75 (4), 798–807. doi: 10.1111/all.14086.
  3. The Diagnosis and Management of Allergic Reactions in Patients Sensitized to Non-Specific Lipid Transfer Proteins. Skypala, I. J.; Bartra, J.; Ebo, D. G.; Antje Faber, M.; Fernández-Rivas, M.; Gomez, F.; Luengo, O.; Till, S. J.; Asero, R.; Barber, D.; Cecchi, L.; Diaz Perales, A.; Hoffmann-Sommergruber, K.; Anna Pastorello, E.; Swoboda, I.; Konstantinopoulos, A. P.; van Ree, R.; Scala, E. Allergy: European Journal of Allergy and Clinical Immunology. Blackwell Publishing Ltd 2021. doi: 10.1111/all.14797.
  4. Oral Mucosa as a Potential Site for Diagnosis and Treatment of Allergic and Autoimmune Diseases. Gomez-Casado, C.; Sanchez-Solares, J.; Izquierdo, E.; Díaz-Perales, A.; Barber, D.; Escribese, M. M. Foods 2021, 10 (5), 970. doi: 10.3390/foods10050970.
  5. Proteomic Profile of Extracellular Vesicles in Anaphylaxis and Their Role in Vascular Permeability. Nuñez‐Borque, E.; Fernandez‐Bravo, S.; Pastor‐Vargas, C.; Alvarez‐Llamas, G.; Gutierrez‐Blazquez, M. D.; Alwashali, E.; Laguna, J. J.; Dionicio, J.; Betancor, D.; Villalobos, V.; Tome‐Amat, J.; Cuesta‐Herranz, J.; Benito‐Martin, A.; Esteban, V. Allergy 2021, all.14792. doi: 10.1111/all.14792.
  6. Increased MiR‐21‐3p and MiR‐487b‐3p Serum Levels during Anaphylactic Reaction in Food Allergic Children. Nuñez‐Borque, E.; Fernandez‐Bravo, S.; Rodriguez Del Rio, P.; Alwashali, E. M.; Lopez‐Dominguez, D.; Gutierrez‐Blazquez, M. D.; Laguna, J. J.; Tome‐Amat, J.; Gallego‐Delgado, J.; Gomez‐Lopez, A.; Betancor, D.; Cuesta‐Herranz, J.; Ibañez‐Sandin, M. D.; Benito‐Martin, A.; Esteban, V. Pediatric Allergy and Immunology 2021, pai.13518. doi: 10.1111/pai.13518.
  7. Retinoic Acid‐loading of the Major Birch Pollen Allergen Bet v 1 May Improve Specific Allergen Immunotherapy: In Silico, in Vitro and in Vivo Data in BALB/c Mice. Hufnagl, K.; Afify, S. M.; Braun, N.; Wagner, S.; Wallner, M.; Hauser, M.; Wiederstein, M.; Gadermaier, G.; Wildner, S.; Redegeld, F. A.; Blokhuis, B. R.; Hofstetter, G.; Pali‐Schöll, I.; Roth‐Walter, F.; Pacios, L. F.; Jensen‐Jarolim, E. Allergy 2020, 75 (8), 2073–2077. doi: 10.1111/all.14259.
  8. Cow’s Milk Protein β-Lactoglobulin Confers Resilience against Allergy by Targeting Complexed Iron into Immune Cells. Roth-Walter, F.; Afify, S. M.; Pacios, L. F.; Blokhuis, B. R.; Redegeld, F.; Regner, A.; Petje, L. M.; Fiocchi, A.; Untersmayr, E.; Dvorak, Z.; Hufnagl, K.; Pali-Schöll, I.; Jensen-Jarolim, E. Journal of Allergy and Clinical Immunology 2021, 147 (1), 321-334.e4. doi: 10.1016/j.jaci.2020.05.023.
  9. Structural Bases for the Allergenicity of Fra a 1.02 in Strawberry Fruits. Orozco-Navarrete, B.; Kaczmarska, Z.; Dupeux, F.; Garrido-Arandia, M.; Pott, D.; Perales, A. D.; Casañal, A.; Marquez, J. A.; Valpuesta, V.; Merchante, C. Journal of Agricultural and Food Chemistry 2020, 68 (39), 10951–10961. doi: 10.1021/acs.jafc.9b05714.
  10. The Key to the Allergenicity of Lipid Transfer Protein (LTP) Ligands: A Structural Characterization. Gonzalez-Klein, Z.; Cuevas-Zuviria, B.; Wangorsch, A.; Hernandez-Ramirez, G.; Pazos-Castro, D.; Romero-Sahagun, A.; Pacios, L. F.; Tome-Amat, J.; Scheurer, S.; Diaz-Perales, A.; Garrido-Arandia, M. Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids 2021, 1866 (7), 158928. doi: 10.1016/j.bbalip.2021.158928.

2020

  1. Association between Flower Stalk Elongation, an Arabidopsis Developmental Trait, and the Subcellular Location and Movement Dynamics of the Nonstructural Protein P3 of Turnip Mosaic Virus. López-González, S.; Navarro, J. A.; Pacios, L. F.; Sardaru, P.; Pallás, V.; Sánchez, F.; Ponz, F. Molecular Plant Pathology 2020, 21 (10), 1271–1286. doi: 10.1111/mpp.12976.
  2. A New Optical Interferometric-Based in Vitro Detection System for the Specific IgE Detection in Serum of the Main Peach Allergen. Espinosa, R. L.; Garrido-Arandia, M.; Romero-Sahagun, A.; Herreros, P.; Tramarin, L.; Laguna, M. F.; Díaz-Perales, A.; Holgado, M. Biosensors and Bioelectronics 2020, 169, 112641. doi: 10.1016/j.bios.2020.112641.
  3. Performance of Basophil Activation Test and Specific IgG4 as Diagnostic Tools in Nonspecific Lipid Transfer Protein Allergy: Antwerp‐Barcelona Comparison. Decuyper, I. I.; Pascal, M.; van Gasse, A. L.; Mertens, C.; Díaz‐Perales, A.; Araujo, G.; Torradeflot, M.; Rius, J.; Balsells, S.; Muñoz‐Cano, R. M.; Bartra, J.; Li, L.; Sabato, V.; Hagendorens, M. M.; Bridts, C. H.; de Clerck, L. S.; Ebo, D. G.; Faber, M. A. Allergy 2020, 75 (3), 616–624. doi: 10.1111/all.14040.
  4. The TNF-like Weak Inducer of the Apoptosis/Fibroblast Growth Factor–Inducible Molecule 14 Axis Mediates Histamine and Platelet-Activating Factor–Induced Subcutaneous Vascular Leakage and Anaphylactic Shock. Mendez-Barbero, N.; Yuste-Montalvo, A.; Nuñez-Borque, E.; Jensen, B. M.; Gutiérrez-Muñoz, C.; Tome-Amat, J.; Garrido-Arandia, M.; Díaz-Perales, A.; Ballesteros-Martinez, C.; Laguna, J. J.; Beitia, J. M.; Poulsen, L. K.; Cuesta-Herranz, J.; Blanco-Colio, L. M.; Esteban, V. Journal of Allergy and Clinical Immunology 2020, 145 (2), 583-596.e6. doi: 10.1016/j.jaci.2019.09.019
  5. Pru p 9, a New Allergen Eliciting Respiratory Symptoms in Subjects Sensitized to Peach Tree Pollen. Blanca, M.; Puche, L. V.; Garrido-Arandia, M.; Martin-Pedraza, L.; Sahagún, A. R.; López-Sánchez, J. D.; Galán, C.; Marin, A.; Villaba, M.; Díaz-Perales, A.; Somoza, M. L. PLoS ONE 2020, 15 (3). doi: 10.1371/journal.pone.0230010.
  6. Are Physicochemical Properties Shaping the Allergenic Potency of Plant Allergens? Costa, J.; Bavaro, S. L.; Benedé, S.; Diaz-Perales, A.; Bueno-Diaz, C.; Gelencser, E.; Klueber, J.; Larré, C.; Lozano-Ojalvo, D.; Lupi, R.; Mafra, I.; Mazzucchelli, G.; Molina, E.; Monaci, L.; Martín-Pedraza, L.; Piras, C.; Rodrigues, P. M.; Roncada, P.; Schrama, D.; Cirkovic-Velickovic, T.; Verhoeckx, K.; Villa, C.; Kuehn, A.; Hoffmann-Sommergruber, K.; Holzhauser, T. Clinical Reviews in Allergy and Immunology. Springer September 2, 2020, pp 1–27. doi: 10.1007/s12016-020-08810-9.
  7. Analytical Model of Electron Density and Its Machine Learning Inference Cuevas-Zuviría, B,; Pacios, L.F. J. Chem. Inf. Model. 2020, 60, 8. doi: 10.1021/acs.jcim.0c00197
  8. A computational structural study on the DNA‑protecting role of the tardigrade‑unique Dsup protein Minguez-Toral M.; Cuevas-Zuviría B.; Garrido-Arandia M.; Pacios L.F. Sci. Rep. 2020. 10:13424 doi: 10.1038/s41598-020-70431-1
  9. Dynamic plasticity of the lipid antigen-binding site of CD1d is crucially favoured by acidic pH and helper proteins Cuevas-Zuviría B.; Mínguez-Toral M.; Díaz-Perales A.; Garrido-Arandia M.; Pacios L.F. Sci. Rep. 2020. 10:5714 doi: 10.1038/s41598-020-62833-y
  10. Structural Dynamics of the Lipid Antigen-Binding Site of CD1d Protein Cuevas-Zuviría B.; Mínguez-Toral M.; Díaz-Perales A.; Garrido-Arandia M.; Pacios L.F. Biomolecules. 2020. 10(4):532 doi: 10.3390/biom10040532
  11. Group 1 allergens, transported by mold spores, induce asthma exacerbation in a mouse model Hernández-Ramírez G.; Pazos-Castro D.; Gómez Torrijos E.; Yuste Montalvo A.; Romero-Sahagun A.; González-Klein Z.; Jimeno-Nogales L.; Escribese MM.; Extremera Ortega A.; Nuñez-Borque E.; Bustamante Orvay L.; Esteban V.; Feo Brito F.; Barber D.; Tome-Amat J.; Garrido-Arandia M.; Díaz-Perales A. Allergy. 2020. doi: 10.1111/all.14347

2019

  1. Pru p 3-Glycodendropeptides Based on Mannoses Promote Changes in the Immunological Properties of Dendritic and T-Cells from LTP-Allergic Patients Palomares F.; Ramos-Soriano J.; Gomez F.; Mascaraque A.; Bogas G.; Perkins JR.; Gonzalez M.; Torres MJ.; Diaz-Perales A.; Rojo J, Mayorga C. Mol Nutr Food Res. 2019 Oct;63(20):e1900553. doi: 10.1002/mnfr.201900553
  2. Structure-Based Multifunctionalization of Flexuous Elongated Viral Nanoparticles. Yuste-Calvo, C.; González-Gamboa, I.; Pacios, L. F.; Sánchez, F.; Ponz, F. ACS Omega 2019, 4 (3), 5019–5028. doi: 10.1021/acsomega.8b02760
  3. Computational Analyses, Molecular Dynamics, and Mutagenesis Studies of Unprocessed Form of [NiFe] Hydrogenase Reveal the Role of Disorder for Efficient Enzyme Maturation. Albareda, M.; Pacios, L. F.; Palacios, J. M. Biochim. Biophys. Acta - Bioenerg. 2019, 1860 (4), 325–340. doi: 10.1016/j.bbabio.2019.01.001.
  4. Effect of Pre- and Post-Weaning Dietary Supplementation with Arginine and Glutamine on Rabbit Performance and Intestinal Health. Delgado, R.; Abad-Guamán, R.; Nicodemus, N.; Diaz-Perales, A.; García, J.; Carabaño, R.; Menoyo, D. BMC Vet. Res. 2019, 15 (1), 199. doi: 10.1186/s12917-019-1945-2.
  5. Transcriptional Profiling of Dendritic Cells in a Mouse Model of Food-Antigen-Induced Anaphylaxis Reveals the Upregulation of Multiple Immune-Related Pathways. Rodriguez, M. J.; Palomares, F.; Bogas, G.; Torres, M. J.; Diaz-Perales, A.; Rojo, J.; Plaza-Seron, M. del C.; Rodriguez-Nogales, A.; Orengo, C.; Mayorga, C.; et al. Mol. Nutr. Food Res. 2019, 63 (3). doi: 10.1002/mnfr.201800759.
  6. Identification and Molecular Characterization of Allergenic Non-Specific Lipid-Transfer Protein from Durum Wheat (Triticum Turgidum). Safi, H.; Wangorsch, A.; Lidholm, J.; Brini, F.; Spiric, J.; Rihs, H. P.; Vieths, S.; Armentia, A.; Farioli, L.; Diaz-Perales, A.; et al. Clin. Exp. Allergy 2019, 49 (1), 120–129. doi: 10.1111/cea.13271.
  7. Glycosylated Nanostructures in Sublingual Immunotherapy Induce Long-Lasting Tolerance in LTP Allergy Mouse Model. Rodriguez, M. J.; Ramos-Soriano, J.; Perkins, J. R.; Mascaraque, A.; Torres, M. J.; Gomez, F.; Diaz-Perales, A.; Rojo, J.; Mayorga, C. Sci. Rep. 2019, 9 (1), 1–9. doi: 10.1038/s41598-019-40114-7.
  8. Inclusion of a Furin Cleavage Site Enhances Antitumor Efficacy against Colorectal Cancer Cells of Ribotoxin α-Sarcin- or RNase T1-Based Immunotoxins. Ruiz-De-La-Herrán, J.; Tomé-Amat, J.; Lázaro-Gorines, R.; Gavilanes, J. G.; Lacadena, J. Toxins (Basel). 2019, 11 (10). doi: 10.3390/toxins11100593.
  9. Interaction of Alt a 1 with SLC22A17 in the Airway Mucosa. Garrido‐Arandia, M.; Tome‐Amat, J.; Pazos‐Castro, D.; Esteban, V.; Escribese, M. M.; Hernández‐Ramírez, G.; Yuste‐Montalvo, A.; Barber, D.; Pacios, L. F.; Díaz‐Perales, A. Allergy 2019, 74 (11), 2167–2180. doi: 10.1111/all.13877.
  10. Peach Tree Pollen and Pru p 9 May Induce Rhinoconjunctivitis and Asthma in Children. Somoza, M. L.; Garrido‐Arandia, M.; Victorio Puche, L.; López‐Sánchez, J. D.; Blanca‐López, N.; Haroun‐Diaz, E.; Romero Sahagún, A.; Díaz‐Perales, A.; Canto, G.; Blanca, M. Pediatr. Allergy Immunol. 2019, 30 (6), pai.13067. doi: 10.1111/pai.13067.
  11. Energy Landscapes of Ligand Motion Inside the Tunnel-Like Cavity of Lipid Transfer Proteins: The Case of the Pru p 3 Allergen. Cuevas-Zuviría, B.; Garrido-Arandia, M.; Díaz-Perales, A.; Pacios, L.F. Int. J. Mol. Sci. 2019, 20, 1432. doi: 10.3390/ijms20061432.

2018

  1. Profilin, a Change in the Paradigm. Rodríguez Del Río P, Díaz-Perales A, Sánchez-García S, Escudero C, Ibáñez MD, Méndez-Brea P, Barber D. J Investig Allergol Clin Immunol. 2018;28(1):1-12. doi: 10.18176/jiaci.0193. Epub 2017 Aug 1.
  2. Current (Food) Allergenic Risk Assessment: Is It Fit for Novel Foods? Status Quo and Identification of Gaps. Mazzucchelli G, Holzhauser T, Cirkovic Velickovic T, Diaz-Perales A, Molina E, Roncada P, Rodrigues P, Verhoeckx K, Hoffmann-Sommergruber K. Mol Nutr Food Res. 2018 Jan;62(1). doi: 10.1002/mnfr.201700278. Epub 2017 Dec 11. Review
  3. Transcriptional Profiling of Dendritic Cells in a Mouse Model of Food-Antigen-Induced Anaphylaxis Reveals the Upregulation of Multiple Immune-Related Pathways. Rodriguez MJ, Palomares F, Bogas G, Torres MJ, Diaz-Perales A, Rojo J, Plaza-Seron MDC, Rodriguez-Nogales A, Orengo C, Mayorga C, Perkins JR. Mol Nutr Food Res. 2018. doi: 10.1002/mnfr.201800759
  4. Non-branched β-1,3-glucan oligosaccharides trigger immune responses in Arabidopsis. Mélida H, Sopeña-Torres S, Bacete L, Garrido-Arandia M, Jordá L, López G, Muñoz-Barrios A, Pacios LF, Molina A. Plant J. 2018 Jan;93(1):34-49. doi: 10.1111/tpj.13755.
  5. Identification and molecular characterization of allergenic non-specific lipid-transfer protein from durum wheat (Triticum turgidum). Safi H, Wangorsch A, Lidholm J, Brini F, Spiric J, Rihs HP, Vieths S, Armentia A, Farioli L, Diaz-Perales A, Pastorello EA, Scheurer S. Clin Exp Allergy. 2018 Sep 10. doi: 10.1111/cea.13271.
  6. Human polyserase-2, a novel enzyme with three tandem serine protease domains in a single polypeptide chain. Cal S, Quesada V, Llamazares M, Díaz-Perales A, Garabaya C, López-Otín C. J Biol Chem. 2018 Jul 27;293(30):11784. doi: 10.1074/jbc.AAC118.004649.
  7. Expression and Interaction Analysis among Saffron ALDHs and Crocetin Dialdehyde. Gómez-Gómez L, Pacios LF, Diaz-Perales A, Garrido-Arandia M, Argandoña J, Rubio-Moraga Á, Ahrazem O. Int J Mol Sci. 2018 May 9;19(5). pii: E1409. doi: 10.3390/ijms19051409.
  8. A Comparative Study of Human Saposins. Garrido-Arandia M, Cuevas-Zuviría B, Díaz-Perales A, Pacios LF. Molecules. 2018 Feb 14;23(2). pii: E422. doi: 10.3390/molecules23020422.
  9. Immunological Changes Induced in Peach Allergy Patients with Systemic Reactions by Pru p 3 Sublingual Immunotherapy. Palomares F, Gomez F, Bogas G, Campo P, Perkins JR, Diaz-Perales A, Rodriguez MJ, Prieto A, Barber D, Torres MJ, Mayorga C. Mol Nutr Food Res. 2018 Feb;62(3). doi: 10.1002/mnfr.201700669. Epub 2018 Jan 8.
  10. Retinoic acid prevents immunogenicity of milk lipocalin Bos d 5 through binding to its immudominant T-cell epitope K. Hufnagl,, D. Ghosh, S. Wagner, A. Fiocchi, L. Dahdah, R. Bianchini, N. Braun, R. Steinborn, M. Hofer, M. Blaschitz, G. A. Roth, G. Hofstetter,F. Roth-Walter, L. F. Pacios, E. Jensen-Jarolim Scientific Reports, 8, Article number 1598/1-12 (2018) doi: 10.1038/s41598-018-19883-0

2017

  1. Profilin, a Change in the Paradigm. Rodríguez Del Río P, Díaz-Perales A, Sánchez-García S, Escudero C, Ibáñez MD, Méndez-Brea P, Barber D. J Investig Allergol Clin Immunol. 2018;28(1):1-12. doi: 10.18176/jiaci.0193. Epub 2017 Aug 1.
  2. Current (Food) Allergenic Risk Assessment: Is It Fit for Novel Foods? Status Quo and Identification of Gaps. Mazzucchelli G, Holzhauser T, Cirkovic Velickovic T, Diaz-Perales A, Molina E, Roncada P, Rodrigues P, Verhoeckx K, Hoffmann-Sommergruber K. Mol Nutr Food Res. 2018 Jan;62(1). doi: 10.1002/mnfr.201700278. Epub 2017 Dec 11. Review.
  3. LPS promotes Th2 dependent sensitisation leading to anaphylaxis in a Pru p 3 mouse model. Rodriguez MJ, Aranda A, Fernandez TD, Cubells-Baeza N, Torres MJ, Gomez F, Palomares F, Perkins JR, Rojo J, Diaz-Perales A, Mayorga C. Sci Rep. 2017 Jan 13;7:40449. doi: 10.1038/srep40449.
  4. Anaphylaxis to hidden potato allergens in a peach and egg allergic boy. Martín-Muñoz MF, Diaz-Perales A, Cannabal J, Quirce S. Eur Ann Allergy Clin Immunol. 2017 Jan;49(1):45-48.
  5. The clinical and immunological effects of Pru p 3 sublingual immunotherapy on peach and peanut allergy in patients with systemic reactions. Gomez F, Bogas G, Gonzalez M, Campo P, Salas M, Diaz-Perales A, Rodriguez MJ, Prieto A, Barber D, Blanca M, Torres MJ, Mayorga C. Clin Exp Allergy. 2017 Mar;47(3):339-350. doi: 10.1111/cea.12901.
  6. Identification of the ligand of Pru p 3, a peach LTP. Cubells-Baeza N, Gómez-Casado C, Tordesillas L, Ramírez-Castillejo C, Garrido-Arandia M, González-Melendi P, Herrero M, Pacios LF, Díaz-Perales A. Plant Mol Biol. 2017 May;94(1-2):33-44. doi: 10.1007/s11103-017-0590-z. Epub 2017 Mar 15.
  7. A relevant IgE-reactive 28kDa protein identified from Salsola kali pollen extract by proteomics is a natural degradation product of an integral 47kDa polygalaturonase. Mas S, Oeo-Santos C, Cuesta-Herranz J, Díaz-Perales A, Colás C, Fernández J, Barber D, Rodríguez R, de Los Ríos V, Barderas R, Villalba M. Biochim Biophys Acta. 2017 Aug;1865(8):1067-1076. doi: 10.1016/j.bbapap.2017.05.007. Epub 2017 May 10.
  8. Multifactorial Modulation of Food-Induced Anaphylaxis. Benedé S, Garrido-Arandia M, Martín-Pedraza L, Bueno C, Díaz-Perales A, Villalba M. Front Immunol. 2017 May 16;8:552. doi: 10.3389/fimmu.2017.00552. eCollection 2017. Review.
  9. Pru p 3-Epitope-based sublingual immunotherapy in a murine model for the treatment of peach allergy. Rodriguez MJ, Mascaraque A, Ramos-Soriano J, Torres MJ, Perkins JR, Gomez F, Garrido-Arandia M, Cubells-Baeza N, Andreu D, Diaz-Perales A, Rojo J, Mayorga C. Mol Nutr Food Res. 2017 Oct;61(10). doi: 10.1002/mnfr.201700110. Epub 2017 Sep 6.
  10. Mechanisms underlying induction of allergic sensitization by Pru p 3. Tordesillas L, Cubells-Baeza N, Gómez-Casado C, Berin C, Esteban V, Barcik W, O'Mahony L, Ramirez C, Pacios LF, Garrido-Arandia M, Díaz-Perales A. Clin Exp Allergy. 2017 Nov;47(11):1398-1408. doi: 10.1111/cea.12962. Epub 2017 Jul 14.
  11. Nut Allergy in Two Different Areas of Spain: Differences in Clinical and Molecular Pattern. Haroun-Díaz E, Azofra J, González-Mancebo E, de Las Heras M, Pastor-Vargas C, Esteban V, Villalba M, Díaz-Perales A, Cuesta-Herranz J. Nutrients. 2017 Aug 21;9(8). pii: E909. doi: 10.3390/nu9080909.
  12. Pleiotropic effects of resistance-breaking mutations on particle stability provide insight on life history evolution in a plant RNA virus Sayanta Bera, Manuel Moreno-Pérez, Sara García-Figuera, Israel Pagan, Aurora Fraile, Luis F. Pacios, Fernando García-Arenal Journal of Virology, 91(18), e00435/1-e00435/17 (2017) doi: 10.1128/JVI.00435-17
  13. Linking iron-deficiency with allergy: role of molecular allergens and the microbiome F. Roth-Walter, Luis F. Pacios, Rodolfo Bianchini, E. Jensen-Jarolim Metallomics, 9(12), 1676-1692 (2017) doi: 10.1039/c7mt00241f

2016

  1. IgE-reactivity profiles to nonspecific lipid transfer proteins in a northwestern European country. Faber MA, Van Gasse AL, Decuyper II, Uyttebroek A, Sabato V, Hagendorens MM, Bridts CH, De Clerck LS, Fernandez-Rivas M, Pascal M, Diaz-Perales A, Ebo DG. J Allergy Clin Immunol. 2017 Feb;139(2):679-682.e5. doi: 10.1016/j.jaci.2016.06.016. Epub 2016 Jul 15.
  2. Characterisation of a flavonoid ligand of the fungal protein Alt a 1. M Garrido-Arandia, J Silva-Navas, C Ramírez-Castillejo, N Cubells-Baeza, C Gómez-Casado, D Barber, JC del Pozo, P González-Melendi, LF Pacios, A Díaz-Perales (2016).. Scientific Reports, 6: Article number 33468/1-9
  3. Is Microarray Analysis Really Useful and Sufficient to Diagnose Nut Allergy in the Mediterranean Area? Goikoetxea MJ, D'Amelio C M, Martínez-Aranguren R, Gamboa P, Garcia BE, Gómez F, Fernández J, Bartra J, Parra A, Alvarado MI, Alonso MI, González E, Terrados S, Moya C, Blanca N, Feo-Brito F, Villalba M, Díaz-Perales A, Sanz ML. J Investig Allergol Clin Immunol. 2016;26(1):31-9
  4. Detection of major food allergens in amniotic fluid: initial allergenic encounter during pregnancy. Pastor-Vargas C, Maroto AS, Díaz-Perales A, Villalba M, Esteban V, Ruiz-Ramos M, de Alba MR, Vivanco F, Cuesta-Herranz J. Pediatr Allergy Immunol. 2016 Nov;27(7):716-720. doi: 10.1111/pai.12608. Epub 2016 Jul 29.
  5. Computational study of pH-dependent oligomerization and ligand binding in Alt a 1, a highly allergenic protein with a unique fold. M Garrido-Arandia, J Bretones, C Gómez-Casado, N Cubells, A Díaz-Perales, LF Pacios (2016). Journal of Computer-Aided Molecular Design, 30:365-379.
  6. Clinical Performance of Commercial ISAC 112 Allergen Microarray Versus Noncommercial RIRAAF Platform for the Diagnosis of Plant Food and Olive Pollen Allergies. Martínez-Aranguren R, Martínez-Botas J, Díaz-Perales A, Villalba M, de la Hoz B, Gómez F, Bartra J, Blanca-López N, Alvarado MI, Moya C, Feo Brito F, Alonso MD, González-Mancebo E, Terrados S, García BE, Gamboa P, Parra A, D Amelio C, Goikoetxea MJ, Sanz ML. J Investig Allergol Clin Immunol. 2016;26(3):185-7. doi: 10.18176/jiaci.00168.
  7. Allergen-Associated Immunomodulators: Modifying Allergy Outcome. Gómez-Casado C, Díaz-Perales A. Arch Immunol Ther Exp (Warsz). 2016 Oct;64(5):339-47. doi: 10.1007/s00005-016-0401-2. Epub 2016 May 13. Review.
  8. Nonsteroidal anti-inflammatory drugs enhance IgE-mediated activation of human basophils in patients with food anaphylaxis dependent and independent of nonsteroidal anti-inflammatory drugs. M Pascal, R Muñoz-Cano, J Milá, ML Sanz, A Díaz-Perales, J Sánchez-López, A García-Moral, M Juan, A Valero, J Yagüe, C Picado, J Bartra (2016). Clinical & Experimental Allergy, 46:1111-1119.
  9. Structural similarities of human and mammalian lipocalins and their function in innate immunity and allergy Erika Jensen-Jarolim, Luis F. Pacios, Rodolfo Bianchini, Gerlinde Hofstetter, Franziska Roth-Walter Allergy, European Journal of Allergy and Clinical Immunology, 71(3), 286-294 (2016) doi: 10.1111/all.12797
  10. A theoretical study on the reaction of ozone with aqueous iodide Óscar Gálvez, M. Teresa Baeza-Romero, Mikel Sanz, Luis F. Pacios Physical Chemistry Chemical Physics, 18(11), 7651-7660 (2016) doi: 10.1039/C5CP06440F
  11. Human management of a wild plant modulates the evolutionary dynamics of a gene determining recessive resistance to virus infection N. Poulicard, L. F. Pacios, J. L. Gallois, D. Piñero, F. García-Arenal, PLoS Genetics, 12(8), e1006214/1-31 (2016) doi: 10.1371/journal.pgen.1006214

2015

  1. Wheat allergy in celiac children. MF Martín-Muñoz, D Rivero, A Díaz-Perales, I Polanco, S Quirce (2015). Pediatric Allergy and Immunology, 27:92-106.
  2. Sensitive detection of major food allergens in breast milk: first gateway for allergenic contact during breastfeeding. C Pastor-Vargas, AS Maroto, A Díaz-Perales, M Villalba, N Casillas-Díaz, F Vivanco, J Cuesta-Herranz (2015). Allergy, 70:1024-1027
  3. Occupational allergic multiorgan disease induced by wheat flour. E Gómez-Torrijos, J Rodríguez-Sánchez, A Díaz-Perales, R García, C García, F Pineda, S Qurice (2015). Journal of Allergy and Clinical Immunology 136:1114-116.
  4. Is the performance of ImmunoCAP ISAC 112 sufficient to diagnose peach and apple allergies?. CM D´Amelio, MJ Goikoetxea, R Martínez-Aranguren, BE García, F Gómez, J Fernández, J Bartra, N Blanca-López, A Díaz-Perales, ML Sanz (2015). Annals of Allergy, Asthma & Immunology, 112: 162-16
  5. Influence of age on IgE response in peanut-allergic children and adolescents from the Mediterranean area. A García-Blanca, A Aranda, N Blanca-López, D Pérez, F. Gómez, C Mayorga, MJ Torres, A Díaz-Perales, JR Perkins, M Villalba, M Blanca, G Canto (2015). Pediatric Allergy and Immunology, 26:497-502.
  6. Impact of Glutathione on the Allergenicity of the Peach Lipid Transfer Protein Pru p 3. C Gómez-Casado, L Tordesillas, J Kinkel, P Starkl, J Cuesta-Herranz, F Roth-Walter, A Díaz-Perales, E Jensen-Jarolim (2015). Journal of Investigational Allergology & Clinical Inmunology, 25:47-54
  7. Immune Polarization in Allergic Patients: Role of the Innate Immune System. MM Escribese, C Gómez-Casado, D Barber, A Díaz-Perales. 2015 Journal of Investigational Allergology & Clinical Inmunology, 25:251-258.
  8. Clinical presentation, allergens, and management of wheat allergy. S Quirce, B Moyano-Martínez, A Díaz-Perales (2015). Expert Review of Clinical Immunology,12:563-572.
  9. Challenges for Allergy Diagnosis in Regions with Complex Pollen Exposures. D Barber, A Díaz-Perales, M Villalba, T Chivato (2015). Current Allergy & Asthma Reports, 15: 496.
  10. Bronchial Challenge With Tri a 14 as an Alternative Diagnostic Test for Baker´s Asthma. A Armentia, M Garrido-Arandia, N Cubells-Baeza, C Gómez-Casado, A Díaz-Perales (2015). Journal of Investigational Allergology & Clinical Inmunology,25:352-357.
  11. A Recombinant Sal k 1 Isoform as an Alternative to the Polymorphic Allergen from Salsola kali Pollen for Allergy Diagnosis. S Mas, P Boissy, RI Monsalve, J Cuesta-Herranz, A Díaz-Perales, J Fernández, C Colás, R Rodríguez, R Barderas, M Villalba (2015). International Archives of Allergy and Immunology, 167:83-93.

2014

  1. High prevalence of lipid transfer protein sensitization in apple allergic patients with systemic symptoms Gomez F, Aranda A, Campo P, Diaz-Perales A, Blanca-Lopez N, Perkins J, Garrido M, Blanca M, Mayorga C, Torres MJ. PLoS One. 2014 Sep 11;9(9):e107304. doi: 10.1371/journal.pone.0107304. eCollection 2014
  2. Immune Suppressive Effect of Cinnamaldehyde Due to Inhibition of Proliferation and Induction of Apoptosis in Immune Cells: Implications in Cancer. F Roth-Walter, A Moskovskich, C Gómez-Casado, A Díaz-Perales, K Oida, J Singer, T Kinaciyan, HC Fuchs, E Jensen-Jarolim (2014). PLoS One, 9, e104802
  3. Characterization of Profilin and Polcalcin Panallergens From Ash Pollen. M Garrido-Arandia, S Mas, E Batanero, A Purohit, G Pauli, R Barderas, M Villalba (2014). Journal of Investigational Allergology & Clinical Inmunology, 5:257-266.
  4. Host resistance selects for traits unrelated to resistance-breaking that affect fitness in a plant virus Aurora Fraile, Jean-Michel Hily, Israel Pagán, Luis F. Pacios, Fernando García-Arenal. Molecular Biology and Evolution, 31(4), 928-939 (2014) doi: 10.1093/molbev/msu045
  5. Alt a 1 from Alternaria interacts with PR5-thaumatin like proteins. Cristina Gómez-Casado, Amaya Murua-García, María Garrido-Arandia, Pablo González-Melendi, Rosa Sánchez-Monge, Domingo Barber, Luis F. Pacios, Araceli Díaz-Perales FEBS Letters, 588(9), 1501-1508 (2014) doi: 10.1016/j.febslet.2014.02.044
  6. Bet v 1 from birch pollen is a lipocalin-like protein acting as allergen only when devoid of iron by promoting Th2 lymphocytes Franziska Roth-Walter, Cristina Gómez-Casado, Luis F. Pacios, Nadine Mothes-Luksch, Georg A. Roth, Josef Singer, Araceli Díaz-Perales, Erika Jensen-Jarolim Journal of Biological Chemistry, 289(25), 17416-17421 (2014) doi:10.1074/jbc.M114.567875
  7. Maturation of Rhizobium leguminosarum hydrogenase in the presence of oxygen requires the interaction of the chaperone HypC and the scaffolding protein HupK Marta Albareda, Luis F. Pacios, Hamid Manyani, Luis Rey, Belén Brito, Juan Imperial, Tomás Ruiz-Argüeso, José M. Palacios Journal of Biological Chemistry, 289(31), 21217-21229 (2014) doi:10.1074/jbc.M114.577403
  8. The major cow milk allergen Bos d 5 manipulates T-helper cells depending on its load with siderophore-bound iron Franziska Roth-Walter, Luis F. Pacios, Cristina Gómez-Casado, Gerlinde Hofstetter, Georg A. Roth, Josef Singer, Araceli Díaz-Perales, Erika Jensen-Jarolim PLoS One, 9(8), e104803/1-8 (2014) doi: 10.1371/journal.pone.0104803
  9. Distortion from Planarity in Arenes Produced by Internal Rotation of One Single Hydroxyl Hydrogen: The Case of Alternariol María Garrido-Arandia, Cristina Gómez-Casado, Araceli Díaz-Perales, Luis F. Pacios Journal of Molecular Graphics and Modelling, 53, 140-147 (2014) doi: 10.1016/j.jmgm.2014.07.011
  10. Molecular Dynamics of major allergens from Alternaria, birch pollen, and peach María Garrido-Arandia, Cristina Gómez-Casado, Araceli Díaz-Perales, Luis F. Pacios Molecular Informatics, 33, 682-694 (2014) doi: 10.1002/minf.201400057
  11. The role of N-glycosylation in kiwi allergy María Garrido-Arandia, Amaya Murua-García, Aranzazu Palacín, Leticia Tordesillas, Cristina Gómez-Casado, Natalia Blanca-López, Tania Ramos, Gabriela Canto, Carlos Blanco, Javier Cuesta-Herranz, Rosa Sánchez-Monge, Luis F. Pacios, Araceli Díaz-Perales Food Science and Nutrition, 2(3), 260-271 (2014) doi: 10.1002/fsn3.99

Communications to congresses

  1. G Hernández-Ramírez, M Garrido-Arandia, J Tomé-Amat, A Romero-Sahagu, LF Pacios, A Díaz Perales. Allergenicity resulting from functional mimicry of a Lipocalin 2. Poster. European Academy of Allergy and Clinical Immunology EAACI, Congress 2018. Munich, 26-30 May, 2018
  2. M. Garrido-Arandia, L Tordesillas, N Cubells-Baeza, V Esteban, W Barcik, Liam O’Mahony, LF. Pacios, A Díaz-Perales. The ligand of the major peach allergen Pru p 3 is presented to iNKT cells. Poster. European Academy of Allergy and Clinical Immunology EAACI, Congress 2018. Munich, 26-30 May, 2018
  3. M Garrido-Arandia, LF Pacios, A Díaz Perales. Major peach allergen Pru p 3 has structural features similar to saposins. Poster. European Academy of Allergy and Clinical Immunology EAACI, Congress 2017. Helsinki, 17-21 June, 2017.
  4. M Garrido-Arandia, N Cubells-Baeza, C Gómez-Casado, L Tordesillas, C Ramírez-Castillejo, LF Pacios, A Díaz Perales. Identification and immunological characterization of the ligand of Pru p 3. Oral Communication. European Academy of Allergy and Clinical Immunology EAACI, Congress 2017. Helsinki, 17-21 June, 2017.
  5. N Cubells-Baeza, W Barcik, M Garrido-Arandia, LF Pacios, A Díaz-Perales, L O´Mahony. Assessment of CD1d presentation of food lipid allergens. Oral communication. Improving Allergy Risk Assessment Strategy for New Food Proteins - IimpARAS International Conference. Varsovia, Polonia, 20 – 22 September 2016
  6. M Garrido-Arandia, J Bretones, C Gómez-Casado, N Cubells-Baeza, A Díaz-Perales, LF Pacios. pH-dependence of oligomerization states and ligand binding in Alt a 1. Oral Comunication. European Academy of Allergy and Clinical Immunology EAACI, Congress 2016. Wien, 11-15 June 2016
  7. N Cubells-Baeza, C Gómez-Casado, M Garrido-Arandia, L Tordesillas, LF Pacios, A Díaz-Perales. Pru p 3-ligand helps inducing Pru p3 sensitization. Poster. Basic Immunology Research in Allergy and Clinical Immunology. 14th Immunology Winter School (EAACI). Pocol, Cortina d'Ampezzo, 4-7 February 2016
  8. N Cubells-Baeza, C Gómez-Casado, L Tordesillas. The role of Pru p 3-ligand in food allergy. Oral comunication. Red de Investigación de Reacciones Adversas a Alérgenos y Fármacos (RIRAAF), Summer School 2015. Málaga, 1 – 2 October2015
  9. M Garrido-Arandia, N Cubells-Baeza, A Díaz-Perales. Alt a 1 sensitization process. Oral communication. Red de Investigación de Reacciones Adversas a Alérgenos y Fármacos (RIRAAF), Summer School 2015. Málaga, 1 – 2 October 2015
  10. N Cubells-Baeza, M Garrido-Arandia, LF Pacios, A Diaz-Perales. N-glycosylation and plant food allergy. Act d 2 as a model. Poster. European Academy of Allergy and Clinical Immunology EAACI, Congress 2015. Barcelona, 6-10 June 2015
  11. M Garrido-Arandia, C Gomez-Casado, F Roth-Walter, N Cubells-Baeza, LF Pacios, J Singer, E Jensen-Jarolim, A Diaz-Perales. The fungal allergen Alt a 1 skews immune cells to Th2 response. e-Poster. European Academy of Allergy and Clinical Immunology EAACI, Congress 2015. Barcelona, 6-10 June 2015
  12. C Gomez-Casado, F Roth-Walter, LF Pacios, N Mothes-Luksch, GA Roth, J Singer, A Diaz-Perales, E Jensen-Jarolim. Bet v 1 is a lipocalin-like allergen capable of binding to siderophore-bound iron thereby skewing T-helper cell responses. Oral communication. European Academy of Allergy and Clinical Immunology EAACI, Congress 2015. Barcelona, 6-10 June 2015

Patents

Methods and means for diagnosis and treating allergy

Inventors
Araceli Díaz Perales, Cristina Gómez Casado, and Luis Fernández Pacios (CBGP-UPM), Josef Singer and Erika Jensen-Jarolim (Medical University of Vienna), Franziska Roth-Walter (Veterinary Medicine University of Vienna)
Type
European patent
Ref
EP14150965.3
Priority date
13/01/2014
Use
Biomedical International R+D GmbH, Vienna, Austria

Composición para el tratamiento de la alergia

Inventors
Araceli Díaz Perales and Luis Fernández Pacios (CBGP-UPM), Cristobalina Mayorga Mayorga, Mª José Torres Jaen, and Miguel Blanca Gómez (Instituto de Investigación Biomédica de Málaga, Servicio Andaluz de Salud), Francisco Javier Rojo Marcos, Ainhoa Mascaraque González, and Francisco Javier Ramos Soriano (CSIC), David Andreu Martínez and Javier Valle García (Universitat Pompeu Fabra)
Type
Spanish patent
Ref
P201531341
Priority date
21/09/2015

PhD Theses

Characterization of the biological activity of Pru P 3 in plant and intestinal epithelium

Name
Nuria Cubells Baeza
Doctorate Program
Biotecnología y Recursos Genéticos de Plantas y Microorganismos Asociados
Year
2017
Abstract
Allergy is a serious social problem that impairs the quality of life of patients and is currently considered the most prevalent chronic disease in Europe. A large increase in its prevalence has occurred since the mid-twentieth century. However, the underlying causes for this increase and the factors that trigger allergy are still unknown. Knowing why a protein becomes an allergen would be essential in the prevention and treatment of allergy diseases. The main aim of this doctoral thesis was to study the molecular mechanisms involved in the allergic sensitization processes. Increasing evidence suggests that lipids transported by certain allergens can be recognized by cellular receptors of the immune system, and may play a prominent role in allergic sensitization. In this work, Pru p 3, the major allergen of the peach and a lipid-transfer protein (LTP), has been chosen as model. The lipid ligand of Pru p 3 has been structurally characterized for the first time using ESI-micrOTOF-QII. The ligand has thus been identified as a hydroxyl derivative of camptothecin bound to phytosphingosine, a hydrocarbon tail which is inserted into the hydrophobic tunnel of the protein. This result was validated by assays of inhibition of topoisomerase I as well as fluorescence emission at 254 nm, both features characteristic of camptothecin. Additionally, different in vitro techniques (such as RT-PCR and immunohistochemistry) were combined tocharacterize the possible role of the ligand in plant. The highest expression of Pru p 3 was detected in pollinated styles by contrast to its absence in non-pollinated flowers where the expression decreased after the antithesis. Besides, the highest expression of Pru p 3 was localized in the tricomas, but not in the pulp. These data, together with the inhibition of pollen germination by the ligand, suggest that Pru p 3 can inhibit a secondary pollination and keep herbivores away until seed maturation. Additionally, the immunological activity of the lipid ligand was evaluated using different in vitro models (moDC, PBMCs, THP1, and Caco2) and a mouse model of anaphylaxis. Thus, it was determined that the ligand was capable of modulating the immune system through a Th2 response, responsible for the activation of antigen presenting cells, and in increasing the capacity of allergic sensitization to Pru p 3 in a mouse model. Furthermore, the ligand was introduced by the CD1d receptor into the epithelial cells to activate iNKT cells. These results have been validated using the ex vivo model InTESTineTM with fragments of pig tissue. The ligand did not affect the transport of Pru p 3 through the intestinal barrier, but appears to remain accumulated in Peyer's Patches. The presence of the ligand was also observed to induce the expression of cytokines that are typical of a Th2 response like IL4 and IL13. Moreover, it was confirmed that Pru p 3 colocalized with the CD1d receptor in the presence of the ligand, as well as increasing the presence of iNKT cells. In summary, this thesis work represents a novel approach to characterize allergic response mechanisms and presents results which are useful for improving diagnosis and for providing a basis for new investigations related to allergy.

Study of the allergenicity of Alt A 1, a unique fungal protein

Name
María Garrido Arandia
Doctorate Program
Biotecnología y Recursos Genéticos de Plantas y Microorganismos Asociados
Year
2016
Abstract
Allergy is a social problem that represents a serious decrease in quality of life of the patients, being considered the biggest chronic disease in Europe today. Since mid-twentieth century, a large increase in the prevalence of these diseases has occurred. However, the underlying causes for this increase and the factors that trigger allergy are still unknown. The knowledge of these factors would be very valuable to prevent and treat allergy disease. The main aim of this doctoral thesis was to study the molecular mechanisms involved in the allergic sensitization processes. With this purpose, the research was focused on Alt a 1, the major allergen from the fungus Alternaria alternata, which has been characterized from structural, physiological, and immunological standpoints. Alt a 1 protein is related to chronic asthma and pathogenesis in plants, although its biological function still remains unknown. However, this allergen has been characterized in this doctoral thesis as an effector which is released when the fungal spores reach the plant surface and is able to inhibit plant defense proteins such as PR5. On the other side, based upon the three-dimensional structure of Alt a 1 obtained in X-ray crystallography, this doctoral thesis also presents an in silico study of the behavior, stability, and properties of several aggregation states of this singular allergen. This study, conducted on salt aqueous solution by means of Molecular Dynamics simulations, has managed to elucidate the dynamical evolution of stabilities, properties, and interactions of the different oligomerization states of Alt a 1 that happened to depend on the presence of ligands and on the pH of the medium. By combining experimental assays in vitro and computational procedures for analyses in silico, the presence of a flavonoid-type ligand bound to Alt a 1 has been also identified in this doctoral thesis. Although it has not been possible to elucidate the precise chemical nature of this ligand, the experimental evidence reported has allowed to propose a plausible structure based on a flavone-like moiety. Finally, the immune response triggered by Alt a 1 in bronchial epithelium was studied. In a similar manner to what happens when Alternaria contacts plants, it has been observed that the presence of fungal spores produces an increase in secretion of defense proteins. Moreover, is has been also observed that Alt a 1 is able to interact with those proteins. The sequence of events that lead to this molecular recognition occur with release of the ligand and promote dimerization of Alt a 1. This dimeric structure is that which is ultimately recognized by antigen presenting cells, thus triggering the allergic response. Summarizing, data presented in this doctoral thesis demonstrate that the knowledge of the biological activity of an allergen provide essential information to better understand its participation in the complex mechanisms that lead to the allergic process.

Sensitization profiles and cross-reactivity among plant allergens. Molecular mechanisms of food allergy development and the role of enhancers

Name
Cristina Gómez Casado
Doctorate Program
Biotecnología y Recursos Genéticos de Plantas y Microorganismos Asociados
Year
2013
Abstract
Allergies are increasing their prevalence from mid twentieth century, and they are currently estimated to affect around 2-8% of the population but the underlying causes of this increase remain still elusive. The understanding of the mechanism by which a harmless protein becomes capable of inducing an allergic response provides us the basis to prevent and treat these diseases. Although the characterization of relevant allergens has led to improved clinical management and has helped to clarify the basic mechanisms of allergic reactions, it seems justified in aspiring to molecularly dissecting these allergens to establish the structural basis of their allergenicity and cross-reactivity. The aim of this thesis was to characterize the molecular basis of the allergenicity of model proteins belonging to different families (Lipid Transfer Proteins ¿LTPs-, and Thaumatin-like Proteins ¿TLPs-) in order to identify mechanisms that mediate sensitization and cross reactivity for developing new strategies in the management of allergy, both diagnosis and treatment, in the near future. With this purpose, two strategies have been conducted: studies of cross-reactivity among panallergen families and molecular studies of the contribution of cofactors in the induction of the allergic response by these panallergens. Following the first strategy, we studied the cross-reactivity among members of two plant panallergens (LTPs , Lipid Transfer Proteins , and TLPs , Thaumatin-like Proteins) using the peach allergy as a model. Similarly, we characterized the sensitization profiles to wheat allergens in baker's asthma development, the most relevant occupational disease. These studies were performed using allergen microarrays and the graph theory for analyzing the results. Regarding the second approach, we analyzed the interaction of plant allergens with immune and epithelial cells. To perform these studies , we examined the importance of ligands and co-transported molecules of plant allergens in the development of Th2 responses. To this end, Pru p 3, nsLTP (non-specific Lipid Transfer Protein) and peach major allergen, was selected as a model to investigate its interaction with cells of the human and murine immune systems as well as with the intestinal epithelium and the contribution of its ligand in inducing an allergic response was studied. Moreover, we analyzed the role of pathogen associated molecules in the induction of food allergy. For that, we selected the kiwi- alternaria system as a model and the role of Alt a 1 , major allergen of the fungus, in the development of Act d 2-sensitization was studied. In summary, this work presents an innovative research providing useful results for improving diagnosis and leading to further research on allergy and the final clarification of the mechanisms that characterize this disease

Molecular basis of cross-reactivity in allergens of plant origin

Name
Leticia Tordesillas Villuendas
Doctorate Program
Biotecnología y recursos genéticos de plantas y microorganismos asociados
Year
2011
Abstract
Allergies have been becoming considerably more widespread since the middle of the last century, but the underlying causes of this increase are still unknown. The knowledge of the mechanism by which a protein that is, in principle, harmless to the human body becomes capable of inducing an allergic response provides us with a basis to prevent and treat these diseases. So far, the only treatment applied in food allergy is the avoidance of consumption of the allergenic source. However, the existence of cross-reactivity among allergens and specific sensitization profiles makes it difficult to predict which foods are related and, therefore, should be avoided by the patient. For the treatment of some allergies, such as bee venom, immunotherapy is yielding good results. Nevertheless, in food allergy, its use is not widespread because of the potential side effects that it may induce, such as anaphylactic shock, arising from the use of non-standardized extracts. To develop safe and effective immunotherapy, it is necessary to characterize the allergens involved at the molecular and immunological levels. LTPs and profilins are two important panallergen families, especially in southern Europe. They are responsible for cross-reactivity among different foods and/or pollen. Within these families, the peach LTP, Pru p 3, and the melon profilin, Cuc m 2, have been characterized as major allergens. The objective of this thesis was to study the molecular basis of the allergic reaction to these proteins in order to identify possible mechanisms that mediate sensitization and cross-reactivity, and to develop safe molecules for generating new strategies in immunotherapy. To this end, the relationship between Pru p 3 and Ole e 7 and Par j 1, LTPs and pollen allergens have been studied to determine whether they are involved in the `LTP syndrome¿. On the other